Norgen Biotek/唾液/拭子RNA纯化96孔试剂盒Dx（Cat。Dx69300）/2x96井板/Dx69300
商品编号：
Dx69300
品牌：
norgenbiotek
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- CE-IVD marked in accordance with EU Directive 98/79/EC
- Fits into in vitro diagnostic workflows
- Isolate high quality total RNA, including viral RNA , from fresh and preserved saliva and swab samples
- High throughput 96-well plate format for rapid purifications
- Fast and easy processing procedure
- Isolate total RNA, from large rRNA down to microRNA (miRNA)
- Very consistent well-to-well RNA isolation
- No phenol or chloroform extractions
- Bind & elute all RNA irrespective of size or GC content, without bias
- Very sensitive & linear down to a few cells/viral copies without the need for carrier RNA
- Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Related Services
NGS
Figure 2. RNase P Gene Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification 96-Well Kit Dx (Cat. #Dx69300).Duplicate preserved (P) and non-preserved (NP) Nasopharyngeal swabs, Oropharyngeal Swabs and Saliva samples were collected from different donors. Preserved Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes Dx (Cat # Dx69300). Preserved Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices Dx (53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification 96-Well Kit Dx, using an input of 0.25 mL from preserved samples or the entire non-preserved swab for the non-preserved conditions. Non-preserved saliva samples were isolated from a 0.25mL input as well. Isolated RNA was tested by amplification of the RNase P gene by RT-PCR. ]">
Figure 3. Human miR-21 Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification 96-Well Kit Dx (Cat. #Dx69300). Duplicate preserved (P) and non-preserved (NP) Nasopharyngeal swabs, Oropharyngeal Swabs and Saliva samples were collected from different donors. Preserved Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes Dx (Cat. #Dx69200). Preserved Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices Dx (Cat # 53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification 96-Well Kit Dx, using an input of 0.25 mL from preserved samples or the entire non-preserved swab for the non-preserved conditions. Non-preserved saliva samples were isolated from a 0.25mL input as well. Isolated RNA was tested by amplification of the human miR-21 detected by stem-loop RT-PCR.]">
RNase P Gene Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgen’s Saliva/Swab RNA Purification 96-Well Kit Dx
Figure 4. Average RNA Yield of Samples Isolated by Norgens Saliva/Swab RNA Purification 96-Well Kit Dx (Cat. #Dx69300). Duplicate preserved (P) and non-preserved (NP) swab (nasal and oral) and saliva samples were collected from different donors. Preserved swab samples (nasal or oral) were collected into Norgens Total Nucleic Acid Preservative Tubes Dx and preserved saliva samples were collected using Norgens Saliva RNA Collection and Preservation Devices (Cat. #53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification 96-Well Kit Dx, using an input of 0.25 mL from preserved samples and the non-preserved saliva or the entire non-preserved swab for the non-preserved swabs. NanoDrop was used to estimate the concentration and yield of the isolated RNA.]">
Human miR-21 Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgen’s Saliva/Swab RNA Purification 96-Well Kit Dx
Figure 1. SARS-CoV-2 E-gene and RdRP Amplification from Preserved Swabs and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification 96-Well Kit (Cat. Dx69300). Duplicate Nasopharyngeal swabs, Oropharyngeal Swabs and Saliva samples were collected from different donors. Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes (Cat. Dx69200). Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices (Cat. 53800). All collected samples were spiked with 3 different concentrations from Norgens E/RdRP/RP Positive Control (included in Norgens COVID-19 TaqMan RT-PCR Kit (E/RdRP genesTM67200) and processed for RNA isolation using Norgens Saliva/Swab RNA Purification 96-Well Kit (Cat. Dx69300), using an input of 0.25 mL from each preserved samples. The sensitivity of the isolation method was assessed amplifying the spiked SARS-CoV-2 spiked targets using Norgens COVID-19 TaqMan RT-PCR Kit (E/RdRP genes (DxTM67200). Figure 1 shows the linear recovery of the different spike-in amounts indicating the high sensitivity of the isolation procedure as well as the high quality of the RNA purified using Norgens Saliva/Swab RNA Purification 96-Well Kit (Cat. Dx69300).]">
Average RNA Yield of Samples Isolated by Norgen’s Saliva/Swab RNA Purification 96-Well Kit Dx
Saliva/Swab RNA Purification 96-Well Kit Dx
Norgen’s Saliva/Swab RNA Purification 96-Well Kit Dx provides a rapid high throughput methodfor the purification of total RNA from non-preserved saliva and nasal/throat swabs, and frompreserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices Dx (Cat.53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes Dx (Cat.Dx69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA ispreferentially purified from other cellular components such as proteins, without the use of phenolor chloroform. The chemistry employed in the kit allows the purification of total RNA, includingviral and bacterial RNA, irrespective of size or GC content. The purified RNA is ideal for in vitrodiagnostic use for medical purposes.
This kit is optimized to be used with any downstream application employing enzymaticamplification or other enzymatic modifications of RNA followed by signal detection oramplification. Any diagnostic results generated using the RNA isolated with Norgen’s Saliva /Swab RNA Purification 96-Well Kit Dx in conjunction with an in vitro diagnostic assay should beinterpreted with regard to other clinical or laboratory findings. To minimize irregularities in diagnostic results, suitable controls for downstream applicationsshould be used. Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx is intended for use by professional userssuch as technicians, physicians and biologists experienced and trained in molecular biologicaltechniques. Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx does not provide a diagnostic result. It isthe sole responsibility of the user to use and validate the kit in conjunction with a downstream invitro diagnostic assay.
Top 5 Reasons to Choose Silicon Carbide RNA Extraction Technology
Saliva RNA Extraction Using Silicon-carbide Technology
品牌介绍
Norgen Achieves Propel Certification
Today on November 20th, 2018, we are proud to announced that we have become Propel-Certified through Illumina as a Next Generation Sequencing (NGS) service provider. Norgen's Propel Certification covers both the NextSeq 500 System and the NextSeq 550 System.
PRNewswire
Norgen Biotek Corp. Named Niagara's Best Business
In October 2012 Norgen Biotek Corporation was named Niagara's company of the year at the 2012 Niagara Business Achievement Awards.
St. Catharines Standard Brock University
Norgen Biotek Corp. Achieves Accreditation to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials
In October 2011 Norgen Biotek Corporation achieved successful certification to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials.
Press release
Gary Goodyear, Minister of State for Science and Technology Visits Norgen Biotek
In February 2011 Gary Goodyear, Minister of State for Science and Technology visited Norgen Biotek Corporation announcing funding to develop two projects to create new diagnostic test kits.
News Video
Norgen Biotek Corp. Achieves ISO 13485:2003 and ISO 9001:2008 Certification
In November 2009 Norgen Biotek Corporation achieved successful certification to ISO 13485:2003 and ISO 9001:2008.
Press Release
NRC-CNRC – Canada’s Innovative Leaders Award
In October 2003, Norgen Biotek was recognized by the National Research Council (NRC) as one of Canada’s Innovative Leaders for the development of their nucleic acid and protein purification technology. NRC recognized the power of Norgen’s technology, and it’s ability to benefit both the genomics and proteomics industries. In December 2007, Norgen Biotek was recognized as one of Canada’s Top 10 Life Science Companies. Canada's Top 10 Life Science Companies are evaluated by an independent international panel of life science venture capitalists. The panel selects companies based on their attractiveness as an exceptional investment in Canada, and Norgen Biotek was chosen as one of these companies. The prestigious Canada’s Top 10 competition was invented in 1999 to promote the ten most promising life science companies chosen from a pool of entrants by an independent international jury of life science venture capitalists. Based on their expert knowledge of investment in the life science sector, the jury selects companies that offer the best investment and partnership prospects.
National Research Council Canada
2002 Niagara Entrepreneur of the Year Awards Bell Canada Emerging Entrepreneur Award
In 2002, Dr. Haj-Ahmad (President and CEO) was nominated for and won the Bell Canada Emerging Entrepreuner Award for his work at Norgen Biotek. The awards are given to men and women who exemplify the true spirit of entrepreneurship, who through hard work, determination, creativity and resourcefulness have established new ventures or lead existing ones into renewed growth.
Niagara Entrepreneur of the Year Awards
