Norgen Biotek/粪便总RNA纯化试剂盒（Cat。49500）/50preps/49500
商品编号：
49500
品牌：
norgenbiotek
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Simultaneous isolation of both host RNA and microbial RNA (universal protocol)
- Isolate full diversity of RNA from large RNA down to small and microRNAs
- Eliminates PCR inhibitors including humic acids
- High quality RNA for sensitive downstream applications
Figure 1. High yields of stool total RNA were purified from 200 mg of human stool using Norgen"s Stool RNA Purification Kit and a leading competitor"s kit, in triplicate. For analysis, 7.5 µL of each 75 µL elution was loaded on a 1.2 % 1x MOPS formaldehyde-agarose gel. Norgen"s kit was found to have a higher yield of RNA, isolated from 200 mg of human infant stool.]">
Stool Total RNA Purification Kit Figure 1
Figure 2. Yield and Quality of Purified Stool RNA. Total RNA was isolated from 200 mg of human stool using Norgen"s Stool RNA Purification Kit and a leading competitor kit. Comparisons were then made based on yield, and A260:A280/A260:A230 ratios measured using the NanoVue Plus™. In Panel A it can be seen that both kits isolated RNA with high A260:A280 ratios (all samples were found to be above 1.8 and below 2.2). In Panel B, Norgen"s kit was found to isolate RNA with a high A260:A230 (with all samples once again falling in the 1.8-2.2 range). The competitor kit, however, was found to isolate RNA with extremely low A260:A230 ratios, with none of the samples displaying a A260:A230 ratio higher than 0.20. The results in Panel C are in agreement with the gel photo from Figure 1, and Norgen"s kit was found to isolate higher amounts of RNA, with an average yield of 14.58 µg, compared to the competitors average of 12.26 µg.]">
Stool Total RNA Purification Kit Figure 2
Figure 3. Detection of Bacterial Stool RNA using 16S Primers. Total stool RNA was isolated from 200 mg human stool samples using Norgen"s Stool RNA Purification Kit and a leading competitors kit. Five microliters of purified RNA was used in a 20 µL reverse-transcription reaction using Invitrogen"s Superscript III system with 16S reverse primers. The cDNA generated was then used in a qPCR reaction involving Norgen"s 2X PCR Mastermix spiked with SYBR green (Bio-Rad), using 0.3µM of primers against bacterial 16S. As can be seen in the amplification plot, Norgen"s kit outperformed the leading competitor"s kit by on average 1.5 Ct values. This indicates that Norgen isolated higher quality and yields of RNA from stool, that can be used in an array of downstream applications.]">
Stool Total RNA Purification Kit Figure 3
Stool Total RNA Purification Kit
This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen"s Stool Nucleic Acid Collection and Transport Tubes (Cat. 45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes.
品牌介绍
Norgen Achieves Propel Certification
Today on November 20th, 2018, we are proud to announced that we have become Propel-Certified through Illumina as a Next Generation Sequencing (NGS) service provider. Norgen's Propel Certification covers both the NextSeq 500 System and the NextSeq 550 System.
PRNewswire
Norgen Biotek Corp. Named Niagara's Best Business
In October 2012 Norgen Biotek Corporation was named Niagara's company of the year at the 2012 Niagara Business Achievement Awards.
St. Catharines Standard Brock University
Norgen Biotek Corp. Achieves Accreditation to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials
In October 2011 Norgen Biotek Corporation achieved successful certification to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials.
Press release
Gary Goodyear, Minister of State for Science and Technology Visits Norgen Biotek
In February 2011 Gary Goodyear, Minister of State for Science and Technology visited Norgen Biotek Corporation announcing funding to develop two projects to create new diagnostic test kits.
News Video
Norgen Biotek Corp. Achieves ISO 13485:2003 and ISO 9001:2008 Certification
In November 2009 Norgen Biotek Corporation achieved successful certification to ISO 13485:2003 and ISO 9001:2008.
Press Release
NRC-CNRC – Canada’s Innovative Leaders Award
In October 2003, Norgen Biotek was recognized by the National Research Council (NRC) as one of Canada’s Innovative Leaders for the development of their nucleic acid and protein purification technology. NRC recognized the power of Norgen’s technology, and it’s ability to benefit both the genomics and proteomics industries. In December 2007, Norgen Biotek was recognized as one of Canada’s Top 10 Life Science Companies. Canada's Top 10 Life Science Companies are evaluated by an independent international panel of life science venture capitalists. The panel selects companies based on their attractiveness as an exceptional investment in Canada, and Norgen Biotek was chosen as one of these companies. The prestigious Canada’s Top 10 competition was invented in 1999 to promote the ten most promising life science companies chosen from a pool of entrants by an independent international jury of life science venture capitalists. Based on their expert knowledge of investment in the life science sector, the jury selects companies that offer the best investment and partnership prospects.
National Research Council Canada
2002 Niagara Entrepreneur of the Year Awards Bell Canada Emerging Entrepreneur Award
In 2002, Dr. Haj-Ahmad (President and CEO) was nominated for and won the Bell Canada Emerging Entrepreuner Award for his work at Norgen Biotek. The awards are given to men and women who exemplify the true spirit of entrepreneurship, who through hard work, determination, creativity and resourcefulness have established new ventures or lead existing ones into renewed growth.
Niagara Entrepreneur of the Year Awards
