Norgen Biotek/白细胞RNA纯化试剂盒（Cat。21250）/50preps/21250
商品编号：
21250
品牌：
norgenbiotek
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Process up to 3 mL of blood and concentrate leukocyte RNA down to 10 to 20 µL
- Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
- No phenol or chloroform extractions
- Isolate total leukocyte RNA, including all small RNA species
- Rapid and convenient spin-column format
- Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen"s NGS services
- Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
Related Services
NGS
RNA Isolation
Figure 1. Higher Yield and Quality of Leukocyte RNA Isolated by Norgen"s Leukocyte RNA Purification Kit. Norgen"s Leukocyte RNA Purification Kit isolates Leukocyte RNA that exceeds the yield and quality of competitors. Total RNA was isolated from 200 µL of hamster blood using Norgen"s Leukocyte RNA Purification Kit and a leading competitors kit. One microliter of the 50 µL purified RNA was resolved on an Agilent RNA Nano 6000 chip. The gel diagram (Upper panel) and the electropherogram (Lower panel) showed better quality of RNA isolated by Norgen"s kit. In particular, RNA isolated by Norgen"s Leukocyte RNA Purification Kit did not have any evidence of the RNA degradationthat was present in RNA isolated by the competitors kit (Red Circle). In addition, Norgen"s kit isolated higher amounts of RNA (Blue Line) with the additional recovery of small RNAs including miRNA (Black Circle) which were not present in RNA isolated by the competitor"s kit. The above observation was further confirmed in RT-qPCR reactions as illustrated in Figure 3.]">
Leukocyte RNA Purification Kit Figure 1
Figure 2. High Yield of a Diversity of RNA Species. Norgen"s Leukocyte RNA Purification Kit effectively recovers all sizes of RNA fromlarge mRNA to small RNA including microRNAs. Total RNA was isolated from 200 µL of hamster blood sample using Norgen"s Leukocyte RNA Purification Kit and a leading competitors kit as illustrated in Figure 2. Two hundred nanograms of the purified RNA (both 50 µL elution volumes) was then used as the template in a RT-qPCR for detecting the beta-Actin gene (Lower Panel) and for detecting miR-21(Upper Panel). In both graphs the blue lines correspond to Norgen isolated-RNA and the red lines correspond to competitor-isolated RNA. As it can be seen, Norgen"s kit isolated higher yields of microRNA, as indicated by the lower Ctvalues of the blue lines (Upper Panel). Also, Norgen"s kit successfully isolated asimilar amount of thelarge RNA compared to the competitors kit (Lower Panel) indicating the full diversity of RNA species isolated.]">
Leukocyte RNA Purification Kit Figure 2
Figure 3. Norgen"sLeukocyte RNA Purification Plus Kit (Cat No. 21250) was used to Isolate RNA from 2.5 mL of Whole Blood Collected from 24 donors, Across 3 Experiments. Donors were tested in duplicate. After the standard protocol failed to produce RNA with sufficient concentration, modifications were made to the procedure and 16 additional donors were tested. 2 batches of 8 additional donors were tested in duplicate with the modified protocol for 32 separate RNA isolations; 32/32 yielded RNA concentration to >100 ng/µL. A single pass of 30 µL of elution buffer was used.
(Tessa Wiehle, Stephanie Borel, Andrew Kincaid, John Hedges, Jon Kemppainen, Justin T Brown, Andrew G Hadd, and Gary J Latham. Modifications to RNA Isolation Protocols Meet Requirements for Modern CML Monitoring of BCR-ABL1 Transcript Levels.AsuragenInc. 2018)
]">
Leukocyte RNA Purification Kit Figure 3
Leukocyte RNA Purification Plus Kit
Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. RNA isolated from blood can be used in various expression studies including those focusing on diseases. However, a major problem with blood RNA isolation is that a large portion of the RNA present is globin mRNA, which is found primarily in red blood cells. In fact, up to 70% of the mass of mRNA in whole blood total RNA is globin transcripts. Therefore, it is desirable to be able to remove the red blood cells from the sample and isolate only the RNA associated with the leukocytes, which will result in improved expression profiling and other applications by removing the masking effects of this abundant globin mRNA. Norgen’s Leukocyte RNA Purification Plus Kit can be used to isolate and purify total leukocyte RNA, including all small RNAs, from mammalian blood samples.
品牌介绍
Norgen Achieves Propel Certification
Today on November 20th, 2018, we are proud to announced that we have become Propel-Certified through Illumina as a Next Generation Sequencing (NGS) service provider. Norgen's Propel Certification covers both the NextSeq 500 System and the NextSeq 550 System.
PRNewswire
Norgen Biotek Corp. Named Niagara's Best Business
In October 2012 Norgen Biotek Corporation was named Niagara's company of the year at the 2012 Niagara Business Achievement Awards.
St. Catharines Standard Brock University
Norgen Biotek Corp. Achieves Accreditation to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials
In October 2011 Norgen Biotek Corporation achieved successful certification to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials.
Press release
Gary Goodyear, Minister of State for Science and Technology Visits Norgen Biotek
In February 2011 Gary Goodyear, Minister of State for Science and Technology visited Norgen Biotek Corporation announcing funding to develop two projects to create new diagnostic test kits.
News Video
Norgen Biotek Corp. Achieves ISO 13485:2003 and ISO 9001:2008 Certification
In November 2009 Norgen Biotek Corporation achieved successful certification to ISO 13485:2003 and ISO 9001:2008.
Press Release
NRC-CNRC – Canada’s Innovative Leaders Award
In October 2003, Norgen Biotek was recognized by the National Research Council (NRC) as one of Canada’s Innovative Leaders for the development of their nucleic acid and protein purification technology. NRC recognized the power of Norgen’s technology, and it’s ability to benefit both the genomics and proteomics industries. In December 2007, Norgen Biotek was recognized as one of Canada’s Top 10 Life Science Companies. Canada's Top 10 Life Science Companies are evaluated by an independent international panel of life science venture capitalists. The panel selects companies based on their attractiveness as an exceptional investment in Canada, and Norgen Biotek was chosen as one of these companies. The prestigious Canada’s Top 10 competition was invented in 1999 to promote the ten most promising life science companies chosen from a pool of entrants by an independent international jury of life science venture capitalists. Based on their expert knowledge of investment in the life science sector, the jury selects companies that offer the best investment and partnership prospects.
National Research Council Canada
2002 Niagara Entrepreneur of the Year Awards Bell Canada Emerging Entrepreneur Award
In 2002, Dr. Haj-Ahmad (President and CEO) was nominated for and won the Bell Canada Emerging Entrepreuner Award for his work at Norgen Biotek. The awards are given to men and women who exemplify the true spirit of entrepreneurship, who through hard work, determination, creativity and resourcefulness have established new ventures or lead existing ones into renewed growth.
Niagara Entrepreneur of the Year Awards
