Norgen Biotek/总RNA纯化试剂盒Dx（Cat。Dx17200）/50preps/Dx17200
商品编号：
Dx17200
品牌：
norgenbiotek
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- CE-IVD marked in accordance with EU Directive 98/79/EC
- Ideal for use in in vitro diagnostic workflows
- Extract high quality & purity total RNA including miRNA
- No phenol step required
- Bind & elute all RNA irrespective of size or GC content, without bias
- Efficiently extract small RNA irrespective of GC content
- Very sensitive & linear down to a few cells without the need for carrier RNA
- Convenient & fast spin column format
- Isolate from a wide variety of specimens
- Purified RNA is suitable for a variety of downstream applications
- Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
Figure 1. High Quality of Isolated RNA with Complete Size Range. Unlike most competitors" kits, Norgen"s Total RNA Purification Kit Dx allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA, without the use of phenol. Total RNA was isolated from 1 x 109 E. coli cells using Norgens Total RNA Purification Kit and a competitors kit. Five microliters and 1 µL of the 50 µL isolated RNA was analyzed on an agarose gel (Panel A) and the Agilent® 2100 BioAnalyzer RNA Nano 6000 chip (Panel B), respectively. Note the presence of small RNA species (red square) in the samples isolated via Norgen"s kit and the absence of these RNA species in the competitor RNA preparation.]">
Figure 1. High Quality of Isolated RNA with Complete Size Range.
Figure 2. Amplification of Both Large and Small RNAs in the Same Extraction. Norgen"s Total RNA Purification Kit Dx isolates the complete size range of RNA, including small RNAs without the use of phenol. Total RNA was isolated from 0.75 million HeLa cells using Norgen"s Total RNA Purification Kit Dx, various silica-based competitors and phenol-based TRI Reagent. RT-PCR was performed according to Shi and Chiang (2005). Fifteen microliters of the 50 µL isolated RNA were polyadenylated in a 50 µL Poly-(A)-Polymerase reaction. Four microliters of the polyadenylated RNA were used in a 20 µL reverse transcription reaction with a poly T adaptor primer. One microliter of the reverse transcription was used in a 20 µL qPCR reaction with primers against the human microRNAs (miR-19 and miR-21) and large mRNA (S15). Only the use of Norgen"s Total RNA Purification Kit Dx resulted in detection of both microRNAs and mRNA amplification products similar to Competitor 2"s kit but without the use of phenol.]">
Figure 2. Amplification of Both Large and Small RNAs in the Same Extraction.
Figure 3. High Quality of RNA from a Diverse Range of Inputs. Norgen"s Total RNA Purification Kit Dx allows RNA isolation from a wide range of species or tissue types. Total RNA was isolated from 5 x 108 E. coli cells, 1 x 106 HeLa cells, 100 µL rat blood and 10 mg hamster kidney using Norgen"s Total RNA Purification Kit Dx. One microliter of the 50 µL isolated RNA was analyzed on the Agilent® 2100 BioAnalyzer using an RNA Nano 6000 chip. Note the integrity of RNA from all inputs with the presence of small RNA species. Norgen"s Total RNA Purification Kit Dx consistently isolates high quality RNA from various inputs that score a RIN value between 8 and 10.]">
Figure 3. High Quality of RNA from a Diverse Range of Inputs.
Figure 4. Great Isolation Sensitivity. Norgen"s Total RNA Purification Kit Dx allows sensitive RNA extraction from as little as a single cell. Total RNA was extracted from a decreasing number of 293 HEK cells from 1 million cells down to a single cell. Five microliters of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using oligo dT primer. Three microliters of the reverse transcription was used in a 20 µL PCR reaction with primers to detect the human beta-actin transcripts. PCR products of beta-actin were detected from as little as a single cell. M is the marker lane.]">
Figure 4. Great Isolation Sensitivity.
Figure 5. Linear and Sensitive Isolation of Both Large and Small RNA. Norgens Total RNA Purification Kit Dx allows consistent isolation of both large and small RNA from different input amounts. Total RNA was isolated from 10 to 100,000 HeLa cells using Norgen"s Total RNA Purification Kit Dx (blue), a competitors silica-based kit (green) and a phenol-based RNA extraction method (red). Relative expression of miR-21 (Panel A), and S15 (Panel B) was determined by RT-qPCR of total RNA samples. In brief, one microliter of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT primer. Two microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21 (Panel A) and the S15transcripts (Panel B). The resulting threshold cycle (Ct) values were plotted against input cell number. RNA isolated using Norgen"s Total RNA Purification Dx had the best linearity (higher R2) and sensitivity (lower Ct) for both large RNA (S15) and small RNA (miR-21).]">
Figure 5. Linear and Sensitive Isolation of Both Large and Small RNA.
Figure 6. Effective and Consistent Detection of miRNA from Plasma. Norgen"s Total RNA Purification Kit Dx can effectively isolate miRNA from plasma. Total RNA was isolated from 50, 100 or 200 µL of rat plasma in triplicates using Norgen"s Total RNA Purification Kit Dx (blue), a competitors silica-based kit (green) and a phenol-based RNA extraction method (red). Stem loop RT-qPCR using primers specific to miR-21 was performed. In brief, two microliters of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT primer. Three microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21. Norgen"s Total RNA Purification Kit Dx is the only product that showed (1) consistent detection of miR-21 transcripts across all input volumes, and (2) Ct values correlated to input volume (decrease Ct with increase input).]">
Figure 6. Effective and Consistent Detection of miRNA from Plasma.
Figure 7. Recovering Diverse miRNA Species from Plasma with Better Consistency. Norgen"s Total RNA Purification Kit Dx isolates plasma RNA that performs more consistently in downstream applications such as microarrays. Total RNA including miRNA was isolated from 100 µL of mouse plasma in duplicate using Norgen"s Total RNA Purification Kit Dx, Competitor 1"s leading miRNA Kit or a phenol-based RNA extraction method. One hundred nanograms of extracted RNA from each kit was applied to an Illumina microRNA expression profiling kit. Scatter plots display better consistency (better clustering) of replicate signals from Norgen"s samples. Genes with Pval < 0.01 for both replicates were in blue. The associated table suggested that Norgen"s protocol recovered the same diversity of miRNA with better consistency (higher r2 value).]">
Figure 7. Recovering Diverse miRNA Species from Plasma with Better Consistency.
Figure 8. Better Diversity of miRNA Detected from Plasma. Norgen"s Total RNA Purification Kit Dx isolates miRNA from plasma with better diversity than a leading competitor. Total RNA including miRNA was isolated from 100 µL of plasma using Norgen"s Total RNA Purification Kit Dx or 625 µL of plasma using Competitor A"s leading miRNA Kit, and was applied to an NCode expression profiling kit. Microarray images suggested that Norgen"s Total RNA Purification Kit Dx (left) isolates a better diversity of miRNA from smaller input amount of plasma than the competitors miRNA kit (right). Image courtesy of LC Sciences, Houston.]">
Figure 8. Better Diversity of miRNA Detected from Plasma.
Figure 9. Better Diversity of miRNA Detected from HeLa Cells using Illumina Small RNA Next Gen Sequencing.Norgen"s Total RNA Purification Kit Dx isolates miRNA from HeLa cells with better diversity than a leading competitor. Total RNA including miRNA was isolated from 1 million HeLa cells using Norgen"s Total RNA Purification Kit Dx and Competitor Q"s leading miRNA Kit, and was applied to Illumina Small RNA Next Gen Sequencing on a MiSeq sequencer. Panel A showed that Norgens Total RNA Purification Kit Dx recovers ahigher number of miRNAsthan the competitor. In particular, the higher diversity is achieved with a faster and simpler procedure in as little as 20 minutes of RNA sample preparation time without the use of phenol. Panel B is a scatter plot of the average RPM (reads per million) of the miRNAs detected used to compare Norgen and the competitorskit recovery of miRNA. Norgens Total RNA Purification Kit Dx recovered a significantly higher number of miRNAs that have higher RPM, as summarized in the graph insert as well as in Panel C.]">
Figure 9. Better Diversity of miRNA Detected from HeLa Cells.
Total RNA Purification Kit Dx
Norgen’s Total RNA Purification Kit Dx provides a rapid method for the isolation and purification of total RNA from tissue samples, blood, plasma, serum, saliva, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use.
This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. Any diagnostic results generated using the RNA isolated with Norgen’s Total RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.
Norgen’s Total RNA Purification Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques.
Norgen’s Total RNA Purification Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
NOTE: This product is not available for sale in the United States.
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品牌介绍
Norgen Achieves Propel Certification
Today on November 20th, 2018, we are proud to announced that we have become Propel-Certified through Illumina as a Next Generation Sequencing (NGS) service provider. Norgen's Propel Certification covers both the NextSeq 500 System and the NextSeq 550 System.
PRNewswire
Norgen Biotek Corp. Named Niagara's Best Business
In October 2012 Norgen Biotek Corporation was named Niagara's company of the year at the 2012 Niagara Business Achievement Awards.
St. Catharines Standard Brock University
Norgen Biotek Corp. Achieves Accreditation to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials
In October 2011 Norgen Biotek Corporation achieved successful certification to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials.
Press release
Gary Goodyear, Minister of State for Science and Technology Visits Norgen Biotek
In February 2011 Gary Goodyear, Minister of State for Science and Technology visited Norgen Biotek Corporation announcing funding to develop two projects to create new diagnostic test kits.
News Video
Norgen Biotek Corp. Achieves ISO 13485:2003 and ISO 9001:2008 Certification
In November 2009 Norgen Biotek Corporation achieved successful certification to ISO 13485:2003 and ISO 9001:2008.
Press Release
NRC-CNRC – Canada’s Innovative Leaders Award
In October 2003, Norgen Biotek was recognized by the National Research Council (NRC) as one of Canada’s Innovative Leaders for the development of their nucleic acid and protein purification technology. NRC recognized the power of Norgen’s technology, and it’s ability to benefit both the genomics and proteomics industries. In December 2007, Norgen Biotek was recognized as one of Canada’s Top 10 Life Science Companies. Canada's Top 10 Life Science Companies are evaluated by an independent international panel of life science venture capitalists. The panel selects companies based on their attractiveness as an exceptional investment in Canada, and Norgen Biotek was chosen as one of these companies. The prestigious Canada’s Top 10 competition was invented in 1999 to promote the ten most promising life science companies chosen from a pool of entrants by an independent international jury of life science venture capitalists. Based on their expert knowledge of investment in the life science sector, the jury selects companies that offer the best investment and partnership prospects.
National Research Council Canada
2002 Niagara Entrepreneur of the Year Awards Bell Canada Emerging Entrepreneur Award
In 2002, Dr. Haj-Ahmad (President and CEO) was nominated for and won the Bell Canada Emerging Entrepreuner Award for his work at Norgen Biotek. The awards are given to men and women who exemplify the true spirit of entrepreneurship, who through hard work, determination, creativity and resourcefulness have established new ventures or lead existing ones into renewed growth.
Niagara Entrepreneur of the Year Awards
