Norgen Biotek/FFPE RNA纯化试剂盒（Cat。25300）/50preps/25300
商品编号：
25300
品牌：
norgenbiotek
市场价：
¥1484.60
美元价：
890.76
产品分类：
逆转录试剂盒
公司分类：
Sensiscript_RT_Kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Extract total RNA (including microRNA) from FFPE samples
- No phenol extraction step
- Includes DNase for optional on-column DNA removal
- Isolated RNA is of the highest quality and integrity
- Isolate a diversity of RNA species
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen"s NGS services
- Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
Related Services
NGS
RNA Isolation
Figure 1. High Quality and Yield of Total RNA. Norgen"s FFPE RNA Purification Kit isolates FFPE RNA that exceeds both yield and quality of competitors. Total RNA was isolated from one slice of hamster FFPE kidney section (20 micron thickness) using Norgen"s FFPE RNA Purification Kit and a leading competitors kit. One microliter of the 50 µL purified RNA was then resolved on an Agilent 2100 BioAnalzyer using an RNA Nano 6000 chip. As it can be seen, Norgen not only isolated higher yields of total RNA, but the RNA was also of a higher quality as evidenced by the higher RIN values obtained with Norgen"s RNA.]">
FFPE RNA Purification Kit Figure 1
Figure 2. Higher Yield of FFPE RNA Isolated by Norgen"s FFPE RNA Purification Kit. Norgen"s FFPE RNA Purification Kit isolates FFPE RNA that exceeds the yield of competitors. Total RNA was isolated from one slice of hamster FFPE kidney section (20 micron thickness) using Norgen"s FFPE RNA Purification Kit and two leading competitor"s kits. Eighteen isolations were performed for each product. The graph demonstrates the mean yield of RNA according to spectrophotometry for 18 sample replicates.Vertical bars represent the standard deviation. Norgen"s kit consistently purified total RNA with a higher yield than for those obtained using the market competitor"s kits.]">
FFPE RNA Purification Kit Figure 2
Figure 3. High Yield of a Diversity of RNA Species. Norgen"s FFPE RNA Purification Kit effectively recovers all sizes of RNA from large mRNA to small RNA including microRNAs. Total RNA was isolated from one slice of hamster FFPE kidney section (20 micron thickness) using Norgens FFPE RNA Purification Kit and a leading competitors kit. Five microliters of the 50 µL purified RNA was then used as the template in a 20 µL reverse transcription reaction with oligo dT primer or miR-21 stem-loop reverse primer. Two microliters of the RT reaction was then used in a 15 µL qPCR reaction for detecting the beta-actin gene (Panel A) and for detecting miR-21(Panel B), respectively. In both graphs the blue lines correspond to Norgen isolated-RNA and the green lines correspond to competitor-isolated RNA. As it can be seen, Norgen"s kit isolated higher yields of RNA in both cases, as indicated by the lower Ct values of the blue lines. Also, Norgen"s kit successfully isolated not only large RNA (Panel A) but also microRNA (Panel B), indicating the diversity of RNA species isolated.]">
FFPE RNA Purification Kit Figure 3
Figure 4. Excellent Performance in Downstream Application such as RT-qPCR. Norgen"s FFPE RNA Purification Kit effectively recovers all sizes of RNA, from large mRNA to small RNA including microRNA, that perform effectively in sensitive applications such as RT-qPCR. Total RNA was isolated from equal amounts of an FFPE tissue sample using Norgen"s FFPE RNA Purification Kit and two market competitors. The graph demonstrates the mean delta Ct value for 18 sample replicates. Vertical bars represent the standard deviation. Norgen"s kit consistently isolates RNA of a higher yield and quality than that obtained by the two leading market competitors as indicated by the larger delta Ct values. Not only were the large RNA species detected including rRNA and mRNA, but small RNA species (microRNA) were also detected from the sample, indicating the diversity of RNA species isolated.]">
FFPE RNA Purification Kit Figure 4
FFPE RNA Purification Kit
Norgen’s FFPE RNA Purification Kit provides a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time.
品牌介绍
Norgen Achieves Propel Certification
Today on November 20th, 2018, we are proud to announced that we have become Propel-Certified through Illumina as a Next Generation Sequencing (NGS) service provider. Norgen's Propel Certification covers both the NextSeq 500 System and the NextSeq 550 System.
PRNewswire
Norgen Biotek Corp. Named Niagara's Best Business
In October 2012 Norgen Biotek Corporation was named Niagara's company of the year at the 2012 Niagara Business Achievement Awards.
St. Catharines Standard Brock University
Norgen Biotek Corp. Achieves Accreditation to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials
In October 2011 Norgen Biotek Corporation achieved successful certification to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials.
Press release
Gary Goodyear, Minister of State for Science and Technology Visits Norgen Biotek
In February 2011 Gary Goodyear, Minister of State for Science and Technology visited Norgen Biotek Corporation announcing funding to develop two projects to create new diagnostic test kits.
News Video
Norgen Biotek Corp. Achieves ISO 13485:2003 and ISO 9001:2008 Certification
In November 2009 Norgen Biotek Corporation achieved successful certification to ISO 13485:2003 and ISO 9001:2008.
Press Release
NRC-CNRC – Canada’s Innovative Leaders Award
In October 2003, Norgen Biotek was recognized by the National Research Council (NRC) as one of Canada’s Innovative Leaders for the development of their nucleic acid and protein purification technology. NRC recognized the power of Norgen’s technology, and it’s ability to benefit both the genomics and proteomics industries. In December 2007, Norgen Biotek was recognized as one of Canada’s Top 10 Life Science Companies. Canada's Top 10 Life Science Companies are evaluated by an independent international panel of life science venture capitalists. The panel selects companies based on their attractiveness as an exceptional investment in Canada, and Norgen Biotek was chosen as one of these companies. The prestigious Canada’s Top 10 competition was invented in 1999 to promote the ten most promising life science companies chosen from a pool of entrants by an independent international jury of life science venture capitalists. Based on their expert knowledge of investment in the life science sector, the jury selects companies that offer the best investment and partnership prospects.
National Research Council Canada
2002 Niagara Entrepreneur of the Year Awards Bell Canada Emerging Entrepreneur Award
In 2002, Dr. Haj-Ahmad (President and CEO) was nominated for and won the Bell Canada Emerging Entrepreuner Award for his work at Norgen Biotek. The awards are given to men and women who exemplify the true spirit of entrepreneurship, who through hard work, determination, creativity and resourcefulness have established new ventures or lead existing ones into renewed growth.
Niagara Entrepreneur of the Year Awards
