Norgen Biotek/microRNA纯化试剂盒（Cat。21300）/25preps/21300
商品编号：
21300
品牌：
norgenbiotek
市场价：
¥3980.00
美元价：
2388.00
产品分类：
特色ELISA试剂盒
公司分类：
Specialty_ELISA_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Extract high quality & purity miRNA in 30 minutes
- Two column kit- eliminate large RNA on first column and capture microRNA on a second column
- Elute microRNA in 25 µL ready for miRNA profiling
- Process a wide range of samples- cell, tissue, bacteria, bodily fluids, etc
- Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
Related Services
NGS
RNA Isolation
Figure 1. High Quality of Isolated RNA with Better Size Exclusion. Small RNA was isolated from HeLa cells using Norgen"s microRNA Purification Kit (Lanes C and D) and a competitor"s kit (Lanes A and B). Ten microliters of the 50 µL purified small RNA were run on an 8% urea-agarose gel. Lane M is Norgen"s 100b RNA ladder. Note that Norgen"s kit is isolating only the small (<200 nt) RNA species, with no contaminating larger RNA.]">
microRNA Purification Kit Figure 1
Figure 2. Efficient Removal of Large RNA Species. Different RNA species were isolated from 106 HeLa cells, resolved on an Agilent® Lab-On-A-Chip and electropherograms were generated. Panel A contains all the RNA species present in 106 HeLa cells as isolated with Norgen"s Total RNA Purification Kit, and acts as a control. Panel B and C contain RNA that was isolated from 106 HeLa cells using Norgens microRNA Purification Kit. One microliter of the 50 µL purified RNA for each fraction was loaded. Panel B shows the large RNA species removed using the Large RNA Removal Columns, and no small RNA can be detected. Panel C shows the small RNA that is isolated using the microRNA Enrichment Columns, and shows that there is no contamination of the small RNA with any large RNA species above 200 nt. This demonstrates the effective separation of the small RNA from the large RNA species using Norgen"s microRNA Purification Kit.]">
microRNA Purification Kit Figure 2
Figure 3. Effective Small RNA Amplification by End-Point RT-PCR. Norgen"s microRNA Purification Kit isolates small RNA that could be amplified in RT-PCR using the poly (A) polymerase extension method. Small RNA was isolated from 1 million HeLa cells, and10µLof the 20 µL isolated small RNA were polyadenylated in a 50 µL Poly-(A)-Polymerase reaction. Seven microliters of the polyadenylated RNA were used in a 20 µL reverse transcription reaction with a poly T adaptor primer. One microliter of the reverse transcription was used in a 20 µL PCR reaction with primers against the human microRNAs (miR-21) and 5S rRNA. Panel A shows the amplification of the miR-21 transcript from small RNAs while Panel B shows the 5S rRNA amplification from small RNAs. Lane 1 in both panels shows the results when total RNAisolated from 1 million HeLa cells using Norgen"s Total RNA Purification Kit was used as a control. Lanes 2 and 3 contain the successful RT-PCR when the microRNA isolated using Norgen"s microRNA Purification Kit was used as the template, and Lanes 4 contain the non-template control. The RT-PCR was successful for both reactions using the microRNA as the template. All PCR products were resolved on a 1X TAE, 3% agarose gel using Norgen"s PCR Ranger asthe molecular weight ladder.]">
microRNA Purification Kit Figure 3
Figure 4. Better Recovery of miRNAs by Norgen"s microRNA Purification Kit. Norgen"s microRNA Purification Kit recovers microRNA more effectively than its competitors. Small RNA was isolated from 0.75 million HeLa cells using Norgen"s microRNA Purification Kit and a competitors"kits. Relative expression of (A) miR-21, (B) miR-19 and (C) 5S rRNA was determined by RT-qPCR of polyadenylated total RNA samples. RT-qPCR was performed according to Shi and Chiang (2005). Fifteen microliters of the 50 µL isolated RNA were polyadenylated in a 50 µL Poly-(A)-Polymerase reaction. Four microliters of the polyadenylated RNA were used in a 20 µL reverse transcription reaction with a poly T adaptor primer. One microliter of the reverse transcription was then used in a 20 µL qPCR reaction with primers against the human microRNAs (miR-19 and miR-21) and house-keeping small RNA (5S rRNA). The resulting threshold cycle (Ct) values were summarized in the graph. Blue = Norgen"s microRNA Purification Kit; orange = Silica-basedCompetitor micro-RNA Kit; green = Silica-basedCompetitor Total RNA Kit; red = Phenol-based reagent protocol; yellow= No Template Control. Norgen"s microRNA Purification Kit recovered more miRNAs (lower Ct) than competitor"s microRNA-specific Kit.]">
microRNA Purification Kit Figure 4
microRNA Purification Kit
This kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured animal cells, small tissue samples, bacterial cells, plants and blood.
Two columns are provided with this kit. The first column captures large RNA, while the small RNA are captured on a second column and are eluted concentrated in 25 µL of nuclease-free water.
The small RNA can be used in various downstream applications relating to miRNA profiling, gene regulation and functional analysis. The eluted RNA is ready for RT-qPCR, microarrays, and NGS applications.
Background
These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation.
品牌介绍
Norgen Achieves Propel Certification
Today on November 20th, 2018, we are proud to announced that we have become Propel-Certified through Illumina as a Next Generation Sequencing (NGS) service provider. Norgen's Propel Certification covers both the NextSeq 500 System and the NextSeq 550 System.
PRNewswire
Norgen Biotek Corp. Named Niagara's Best Business
In October 2012 Norgen Biotek Corporation was named Niagara's company of the year at the 2012 Niagara Business Achievement Awards.
St. Catharines Standard Brock University
Norgen Biotek Corp. Achieves Accreditation to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials
In October 2011 Norgen Biotek Corporation achieved successful certification to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials.
Press release
Gary Goodyear, Minister of State for Science and Technology Visits Norgen Biotek
In February 2011 Gary Goodyear, Minister of State for Science and Technology visited Norgen Biotek Corporation announcing funding to develop two projects to create new diagnostic test kits.
News Video
Norgen Biotek Corp. Achieves ISO 13485:2003 and ISO 9001:2008 Certification
In November 2009 Norgen Biotek Corporation achieved successful certification to ISO 13485:2003 and ISO 9001:2008.
Press Release
NRC-CNRC – Canada’s Innovative Leaders Award
In October 2003, Norgen Biotek was recognized by the National Research Council (NRC) as one of Canada’s Innovative Leaders for the development of their nucleic acid and protein purification technology. NRC recognized the power of Norgen’s technology, and it’s ability to benefit both the genomics and proteomics industries. In December 2007, Norgen Biotek was recognized as one of Canada’s Top 10 Life Science Companies. Canada's Top 10 Life Science Companies are evaluated by an independent international panel of life science venture capitalists. The panel selects companies based on their attractiveness as an exceptional investment in Canada, and Norgen Biotek was chosen as one of these companies. The prestigious Canada’s Top 10 competition was invented in 1999 to promote the ten most promising life science companies chosen from a pool of entrants by an independent international jury of life science venture capitalists. Based on their expert knowledge of investment in the life science sector, the jury selects companies that offer the best investment and partnership prospects.
National Research Council Canada
2002 Niagara Entrepreneur of the Year Awards Bell Canada Emerging Entrepreneur Award
In 2002, Dr. Haj-Ahmad (President and CEO) was nominated for and won the Bell Canada Emerging Entrepreuner Award for his work at Norgen Biotek. The awards are given to men and women who exemplify the true spirit of entrepreneurship, who through hard work, determination, creativity and resourcefulness have established new ventures or lead existing ones into renewed growth.
Niagara Entrepreneur of the Year Awards
