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当前位置: 首页 > 产品中心 > peptide > 诺根生物科技/蛋白质自旋™ 包涵体蛋白分离试剂盒(Cat。17700)/4个代表/17700
商品详细诺根生物科技/蛋白质自旋™ 包涵体蛋白分离试剂盒(Cat。17700)/4个代表/17700
诺根生物科技/蛋白质自旋™ 包涵体蛋白分离试剂盒(Cat。17700)/4个代表/17700
诺根生物科技/蛋白质自旋™ 包涵体蛋白分离试剂盒(Cat。17700)/4个代表/17700
商品编号: 17700
品牌: norgenbiotek
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

  • All-in-one solution for inclusion body protein isolation and purification
  • Complete kit with Cell Lysis Reagent, Inclusion Body Solubilization Reagent, buffers and spin columns to purify proteins
  • Isolate up to 12 mg of protein per spin column
  • Fast and convenient spin column protocol


ProteoSpin™ Inclusion Body Protein Isolation Maxi Kit

This kit provides everything required to isolate and purify inclusion body proteins from induced bacterial cultures. First a proprietary Cell Lysis Reagent is used to selectively lyse the cells and release inclusion bodies in their solid form. Next, inclusion bodies are dissolved and their contents released using the provided IB Solubilization Reagent. Inclusion body proteins are then further purified using spin columns for rapid and convenient buffer exchange and desalting.

The procedure is efficient and streamlined and can process up to 4 samples in approximately 2 hours. Each spin column is able to recover up to 12 mg of acidic or basic proteins from 100 mL of induced bacterial culture.  Purified recombinant proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.

About Inclusion Bodies

Bacteria are widely used for the expression of different proteins. However, 70-80% of the proteins expressed in bacteria by recombinant techniques are tyically contained in insoluble inclusion bodies (i.e., protein aggregates). The protein of interest found in these sub-cellular structures is often inactive, due to incorrect folding. The production rate of recombinant proteins stored in inclusion bodies is invariably higher than those synthesized as soluble proteins. The reason behind this is thought to be the resistance of insoluble proteins to proteolysis by cellular enzymes. In addition, separation of insoluble recombinant proteins in inclusion bodies is considerably easier than that of soluble proteins. These factors have been the major influences favoring scale-up of high-value proteins using bacterial fermentation for example.  Procedures for the purification of the expressed proteins from inclusion bodies are often labour-intensive, time consuming and not cost effective.   This kit provides the essential reagents for cell disruption, inclusion body solubilization and purification using spin column chromatography - all optimized to work together thereby simplifying the process and saving a tremendous amount of time and cost.  

 

 

品牌介绍
Norgen Achieves Propel Certification Today on November 20th, 2018, we are proud to announced that we have become Propel-Certified through Illumina as a Next Generation Sequencing (NGS) service provider. Norgen's Propel Certification covers both the NextSeq 500 System and the NextSeq 550 System. PRNewswire Norgen Biotek Corp. Named Niagara's Best Business In October 2012 Norgen Biotek Corporation was named Niagara's company of the year at the 2012 Niagara Business Achievement Awards. St. Catharines Standard Brock University Norgen Biotek Corp. Achieves Accreditation to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials In October 2011 Norgen Biotek Corporation achieved successful certification to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials. Press release Gary Goodyear, Minister of State for Science and Technology Visits Norgen Biotek In February 2011 Gary Goodyear, Minister of State for Science and Technology visited Norgen Biotek Corporation announcing funding to develop two projects to create new diagnostic test kits. News Video Norgen Biotek Corp. Achieves ISO 13485:2003 and ISO 9001:2008 Certification In November 2009 Norgen Biotek Corporation achieved successful certification to ISO 13485:2003 and ISO 9001:2008.  Press Release NRC-CNRC – Canada’s Innovative Leaders Award In October 2003, Norgen Biotek was recognized by the National Research Council (NRC) as one of Canada’s Innovative Leaders for the development of their nucleic acid and protein purification technology. NRC recognized the power of Norgen’s technology, and it’s ability to benefit both the genomics and proteomics industries. In December 2007, Norgen Biotek was recognized as one of Canada’s Top 10 Life Science Companies. Canada's Top 10 Life Science Companies are evaluated by an independent international panel of life science venture capitalists. The panel selects companies based on their attractiveness as an exceptional investment in Canada, and Norgen Biotek was chosen as one of these companies. The prestigious Canada’s Top 10 competition was invented in 1999 to promote the ten most promising life science companies chosen from a pool of entrants by an independent international jury of life science venture capitalists. Based on their expert knowledge of investment in the life science sector, the jury selects companies that offer the best investment and partnership prospects. National Research Council Canada 2002 Niagara Entrepreneur of the Year Awards Bell Canada Emerging Entrepreneur Award In 2002, Dr. Haj-Ahmad (President and CEO) was nominated for and won the Bell Canada Emerging Entrepreuner Award for his work at Norgen Biotek. The awards are given to men and women who exemplify the true spirit of entrepreneurship, who through hard work, determination, creativity and resourcefulness have established new ventures or lead existing ones into renewed growth. Niagara Entrepreneur of the Year Awards